5. (Prof. JW Lee) Genetic circuit engineering and cell-free synthetic biology for molecular diagnosis and genetic part p

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5 Prof Jw Lee Genetic Circuit Engineering And Cell Free Synthetic Biology For Molecular Diagnosis And Genetic Part P 1 (85.37 KiB) Viewed 86 times

5. (Prof. JW Lee) Genetic circuit engineering and cell-free synthetic biology for molecular diagnosis and genetic part prototyping A. Compare and contrast 'toggle' vs 'deadman' switches. Discuss application of monostable toggle switch for genetic biocontainment. B. Prof. Lee discussed a CRISPR/Cas12a-based assay coupled with cyanobacteria cell-free systems to rapidly prototype promoter characteristics. His group demonstrated cyanobacteria cell-free transcription and confirmed a positive correlation between the in vitro and in vivo transcription performance. Why was the cell-free system useful for cyabobacteria? C. They employed an RNA-guided endonuclease, CRISPR/Cas12a which shows trans-cleavage activity toward neighboring random ssDNAs when forming a complex with its CRISPR RNA (crRNA) and the crRNA-complementary ssDNA or dsDNA. Explain how this endonuclease activity is linked with promoter strength in vitro. +