Figure Below Details The Properties Of Four Proteins Which Are Translocated Across The Endoplasmic Reticulum Er Membra 1 (78.08 KiB) Viewed 50 times
Figure Below Details The Properties Of Four Proteins Which Are Translocated Across The Endoplasmic Reticulum Er Membra 2 (15.54 KiB) Viewed 50 times
Figure below details the properties of four proteins which are translocated across the Endoplasmic Reticulum (ER) membrane by the Sec translocator. These proteins may either be soluble or transmembrane proteins. Protein 1 N C Protein 2 N + Protein 3 N + Protein 4 N C Polypeptide segment Negatively charged polypeptide segment Hydrophobic Polypeptide segment + Positively charged polypeptide segment Figure. Diagrammatic representations of the polypeptide sequence properties of four proteins. Positively/Negatively charged regions are indicated by +/- and hydrophobic regions corresponding to either signal sequences or start/stop transfer sequences are shown in red. Answer the following questions: (a) For Protein 2 is the N-terminus or C-terminus predicted to reside in the cytosol? Explain your reasoning! [20%] (b) For Protein 3 describe the topology of the protein in the membrane including which side of the membrane the N-terminus and C-terminus are located. Explain your reasoning. [25%] (c) Which of the four proteins is least likely to be a transmembrane protein? Explain your reasoning. [15%] d Kingdom) 33 C C
(d) How many transmembrane helices is Protein 4 predicted to possess? Explain your reasoning. [15%] (e) For Protein 4 is the C-terminus predicted to reside in the cytosol or ER lumen? Explain your reasoning. [25%] T
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