
IN ANSWERING QUESTIONS 1-4, REFER TO THE STRUCTURE OF THE PENTAPEPTIDE SHOWN BELOW IN NEUTRAL FORM AND THE DATASHEET PROVIDED: The pK₂ values of the ionisable groups on this peptide are: Corbergu i. a-COOH = 2.1 NH₂ ii. a-NH2 = 9.1 HO OH 1. What is the primary sequence of the pentapeptide using the three-letter amino acid code? A. Met-Phe-Gly-Gly-Tyr B. Tyr-Gly-Gly-Phe-Met C. Phe-Gly-Gly-Tyr-Met D. Cys-Tyr-Gly-Gly-Phe E. Met-Tyr-Gly-Gly-Phe 2. At pH 7, what will be the total charge on this pentapeptide? A. 0.0 B. 0.5 C. 1.0 D. 1.5 E. 2.0 3. From the list of proteolytic enzymes provided below, choose one that will cleave this pentapeptide: A. Chymotrypsin. B. Trypsin. C. Pepsin. D. Elastase. E. Endopeptidase V8. 4. Carboxypeptidase is an enzyme that will cut this peptide: A. after lysine (K). B. after any aromatic residue. C. after the first amino acid residue. D. before the last amino acid residue. E. after methionine (M).