You are working in Prof Sayed's lab and you are tasked with the isolation and characterisation of a novel HIV-1 mutant p

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You Are Working In Prof Sayed S Lab And You Are Tasked With The Isolation And Characterisation Of A Novel Hiv 1 Mutant P 1 (30.92 KiB) Viewed 57 times

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You Are Working In Prof Sayed S Lab And You Are Tasked With The Isolation And Characterisation Of A Novel Hiv 1 Mutant P 3 (30.48 KiB) Viewed 57 times

You are working in Prof Sayed's lab and you are tasked with the isolation and characterisation of a novel HIV-1 mutant protease found in patients failing protease inhibitor drug treatment. The data in the table below were collected from initial reaction rates of the mutant protease-catalysed reaction at the substrate concentrations as shown. Answer the questions that follow. (M/min) [5] (mole/liter) 130 65 x 10-5 116 23 x 10-5 87 7.9 x 10- 63 3.9 x 10-5 30 1.3 x 10-5 10 0.37 x 10 2.1 State two techniques that can be used to characterise the mutant HIV protease. (1 mark) 2.2 Use sound reasoning to justify why you selected the two techniques above. (2 marks) 2.3 Before you can perform enzyme activity measurements, it is necessary to identify a suitable substrate for the mutant HIV protease. Briefly discuss how you will accomplish this task. (3 marks) 2.4 The design of any enzyme assay requires knowledge of the reaction that occurs between an enzyme and a substrate. Briefly state the most important information needed when designing an enzyme assay. (3 marks) 2.5 Determine the K and V values using a suitable graphical method. You must submit your answers together with a detailed and clearly labelled graph indicating how these values were obtained. Merely reporting the Ku and V values are not acceptable. (4 marks) 2.6 if the original concentration of mutant HIV protease is 2.8 μmol/L, calculate the kat (sec) of the mutant HIV protease. (2 marks)

You are working in Prof Sayed's lab and you are tasked with the isolation and characterisation of a novel HIV-1 mutant protease found in patients falling protease inhibitor drug treatment The data in the table below were collected from initial reaction rates of the mutant protease-catalysed reaction at the substrate concentrations as shown. Answer the questions that follow (M/min) [S] (mole liter) 130 65 x 105 116 23 × 10 87 79 x 10-5 3.9 × 10-9 30 1.3 x 10 10 0.37 x 10 2.1 State two techniques that can be used to characterise the mutant HIV protease. (1 mark) 2.2 Use sound reasoning to justify why you selected the two techniques above. (2 marks) 2.3 Before you can perform enzyme activity measurements, it is necessary to identify a suitable substrate for the mutant HIV protease Briefly discuss how you will accomplish this task (3 marks) 2.4 The design of any enzyme assay requires knowledge of the reaction that occurs between an enzyme and a substrate. Briefly state the most important information needed when designing an enzyme (3 marks) assay 2.5 Determine the K and V values using a suitable graphical method. You must submit your answers together with a detailed and clearly labelled graph indicating how these values were obtained Merely reporting the Ku and V values are not acceptable. (4 marks) 2.6 if the original concentration of mutant HIV protease is 2.8 μmol/L, calculate the ken (sec) of the mutant HIV protease (2 marks)