- 1 (21.56 KiB) Viewed 44 times
4. You want to dilute a culture of E. coli at a concentration of 2-10° cells/ml to a point where you can spread 100 μl o
-
answerhappygod
- Site Admin
- Posts: 899603
- Joined: Mon Aug 02, 2021 8:13 am
4. You want to dilute a culture of E. coli at a concentration of 2-10° cells/ml to a point where you can spread 100 μl o
4. You want to dilute a culture of E. coli at a concentration of 2-10° cells/ml to a point where you can spread 100 μl on a plate and get a countable number of colonies (define "countable" as 50 to 300). 4a. What volume of the original culture would you need to pipette into 100 µl fresh growth medium in order to get a countable number of colonies? 4b. Considering that the smallest volume you can pipet accurately is about 2 µl, can you perform the dilution calculated in 4a?