Oxygen consumed ----- Time 1 2 3 STEPS 4A Step 1. Succinate is added 4B Step 2. ADP + Pi is added 4C Step 3. The compoun

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Oxygen Consumed Time 1 2 3 Steps 4a Step 1 Succinate Is Added 4b Step 2 Adp Pi Is Added 4c Step 3 The Compoun 1 (151.52 KiB) Viewed 34 times

Oxygen consumed ----- Time 1 2 3 STEPS 4A Step 1. Succinate is added 4B Step 2. ADP + Pi is added 4C Step 3. The compound FCCP (Carbonyl-p- trifluoromethoxyphenylhrdrazone) is added. FCCP carries protons across the inner-membrane and dissipates any proton gradient. 4D Step 4. Rotenone is added. Rotenone inhibits the transfer of electrons to ubiquinone by complex I (the NADH:ubiquinone oxidoreductase complex) 4 Finish Experiment EXPLANATION ATP Synthesized Question #4 The graph on the left shows data for an experiment in which isolated mitochondria were suspended in buffered medium. In the experiment the ability of the mitochondria to consume oxygen (dashed lines) and produce ATP (solid lines) was monitored after the addition of various compounds. Complete the graph to show what happens (how the oxygen consumption and ATP production of the mitochondria change) at steps 1 to 4 in the experiment. In the boxes below briefly explain your answer for each step.