17 pts The Lineweaver-Burk plot (1/Vvs 1/15) shown below is a common way to plot Michaelis-Menten data in a linear form

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17 Pts The Lineweaver Burk Plot 1 Vvs 1 15 Shown Below Is A Common Way To Plot Michaelis Menten Data In A Linear Form 1 (29.55 KiB) Viewed 31 times

17 pts The Lineweaver-Burk plot (1/Vvs 1/15) shown below is a common way to plot Michaelis-Menten data in a linear form in order to determine the kinetic constants V and 1/pem Intercept-/ I Intercept 1/ VISE a) (4 pts) Calculate the values of Vas (M/min) and KM (UM) for a specific enzyme if the equation for the Lineweaver-Burk plot fit to the data is y-0.224x+0.0222 Enter the answer on the exam, show all your calculations for full credit on the worksheet. Use units where appropriate. bi (4 pts) Calculate the values of Vax (uM/min) and Kse (uM) for the same enzyme in the presence of an inhibitor, if the equation for the Lineweaver-Burk plot fit to the data is y -0.6663x +0.0222. Enter the answer on the exam, show all your calculations for full credit on the worksheet. Use units where appropriate. c) (2 pts) Based on the data, what type of inhibition is this? Explain your answer. d) (7 pts) Calculate kear (in s¹) for this enzyme in the absence of inhibitor, if the above data is obtained in the presence of 75 nM enzyme and the enzyme has 2 active sites. Be careful with unit conversions! Enter the answer on the exam, show all your calculations for full credit on the worksheet. Use units where appropriate. Edit Format Table To B 12pt Paragraph B I U T²4