1. In the HPLC analysis of caffeine in an energy drink by standard addition, the sample was prepared by pipetting 5.00 m

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1 In The Hplc Analysis Of Caffeine In An Energy Drink By Standard Addition The Sample Was Prepared By Pipetting 5 00 M 1 (63.63 KiB) Viewed 129 times

1. In the HPLC analysis of caffeine in an energy drink by standard addition, the sample was prepared by pipetting 5.00 mL of the drink into a 50 ml volumetric flask, adding a fixed volume of a 1.205 mg/mL caffeine standard solution, and then diluting to volume using the HPLC mobile phase. The table summarizes the sample preparation protocol and the results obtained from the three injections: High-Performance Liquid Chromatography Data for an Energy Drink VOLUME OF HEIGHT OF CAFFEINE CAFFEINE PEAK IN VOLUME OF ENERGY STANDARD FINAL DILUTION CHROMATOGRAM DRINK ADDED (mL) ADDED (mL) VOLUME (mL) (ARBITRARY UNITS) 5.00 0.00 50.00 24.79 5.00 0.50 50,00 46.77 5.00 2.00 50,00 80.40 AREA OF CAFFEINE PEAK IN CHROMATOGRAM (ARBITRARY UNITS) 408.3 831.7 1278.6 (a) Use the peak height data to estimate the original concentration of caffeine in the energy drink. (b) Use the peak area data to estimate the original concentration of caffeine in the energy drink. (c) Ofthe two values calculated in (a) and (b), which would you consider more reliable? Explain your answer.