12. Jessica bought a tamago-yaki (fried scrambled egg) and decided to isolate the lipids from it through solvent extract

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12 Jessica Bought A Tamago Yaki Fried Scrambled Egg And Decided To Isolate The Lipids From It Through Solvent Extract 1 (93.18 KiB) Viewed 14 times

12. Jessica bought a tamago-yaki (fried scrambled egg) and decided to isolate the lipids from it through solvent extraction. After obtaining the organic layer, she added acetone until formation of precipitate stopped. She performed several tests on the precipitate and supernatant she obtained after centrifugation. 12.1.Fill in the table below with the result of each of the qualitative tests performed on the obtained precipitate and supernatant. Test Precipitate Supernatant Sudan IV Red color Acrolein test Test for Phosphates No visible change Leibermann- Burchard Test Black solution 12.2. Jessica wanted to know what phospholipids are found in her tamago-yaki. Using thin-layer chromatography, Jessica analyzed her lipid extract. She used the following standards: A: cardiolipin B: phosphatidylethanolamine C: phosphatidylserine D: phosphatidylcholine -SOLVENT FRONT - SAMPLE BLOTS A B Ċ S D She obtained the chromatogram via iodine staining shown in the right (S is for the lipid extract). Determine the major phospholipids Jessica was able to extract from her tamago-yaki using retention factors