Biochemistry Lab Report - Blast Searches (Pictures of results
attached) - 1) from Blastn search 2) from Blastx search.
● Compare the top scores and e-values of the BLASTn and BLASTx
searches of your assigned DNA sequence. Which returned the highest
score? Lowest e-value? explain why.
Please be sure to compare both BLASTn and BLASTx from the assigned
sequence. Why searching protein database will lead to high or
low score? E value?
● How would your score and e-value be affected if you performed
a search with the same percentage identity as your sequence of
interest, but with a shorter query sequence Explain why.
- Biochemistry Lab Report Blast Searches Pictures Of Results Attached 1 From Blastn Search 2 From Blastx Search 1 (163.49 KiB) Viewed 12 times
Descriptions Graphic Summary Alignments Taxonomy V Sequences producing significant alignments Select columns Show select all 100 sequences selected Graphics Distance tree of results MSA Viewer Description Scientific Name Max Total Query E Per. Acc. Score Score Cover value Ident Len ▼ Accession ▼ ♥ ♥ ♥ PREDICTED: Ailuropoda melanoleuca fructose-bisphosphate aldolase A (LOC117800214), mRNA PREDICTED: Chelonoidis abingdonii fructose-bisphosphate aldolase A (LOC116819368) mRNA ✔Homo sapiens aldolase, fructose-bisphosphate A (ALDOA), transcript variant 6. mRNA ✔Homo sapiens aldolase, fructose-bisphosphate A (ALDOA), transcript variant 2, mRNA ✔Homo sapiens uncharaterized LOC112694756 (LOC112694756), transcript variant 3. mRNA ✔Homo sapiens aldolase A fructose-bisphosphate, mRNA (CDNA clone MGC:17716 IMAGE:3869603) compl... Homo sapiens ✔Human ORFeome Gateway entry vector pENTR223-ALDOA, complete sequence Human ORFeo... ✔Synthetic Ailuropoda mela... 217 217 100% 2e-52 100.00% 1219 XM_034652746.1 Chelonoidis abi... 217 217 100% 2e-52 100.00% 1180 XM_032771057.1 Homo sapiens 217 217 100% 2e-52 100.00% 1640 NM 001243177.4 Homo sapiens 217 217 100% 2e-52 100.00% 1486 NM 184041.5 Homo sapiens 217 217 100% 217 217 100% 217 217 100% construct Homo sapiens clone CCSBHm_00000711 ALDOA (ALDOA) mRNA encodes complete... synthetic construct 217 217 100% ✔Synthetic construct Homo sapiens clone CCSBHm_00000709 ALDOA (ALDOA) mRNA encodes complete... synthetic construct 217 217 100% ✔Synthetic construct Homo sapiens clone ccsbBroadEn_00053 ALDOA gene encodes complete protein synthetic construct 217 217 100% Apostichopus ja... 217 217 100% Homo sapiens 217 217 100% Homo sapiens 217 217 100% Homo sapiens 217 217 100% Homo sapiens 217 217 100% 2e-52 100.00% 2569 NM_001365307.2 2e-52 100.00% 1505 BC010660.2 2e-52 100.00% 3882 LT741211.1 2e-52 100.00% 1224 KR709337.1 2e-52 100.00% 1224 KR709336.1 2e-52 100.00% 1224 KJ890659.1 2e-52 100.00% 1382 JF796138.1 2e-52 100.00% 2425 NM_001365305.2 2e-52 100.00% 2323 NM_001365304.2 2e-52 100.00% 1630 NM 001127617.2 2e-52 100.00% 1569 NM 184043.2 Apostichopus japonicus aldolase class-1 protein (ALDOA) mRNA, complete cds Homo sapiens uncharaterized LOC112694756 (LOC112694756), transcript variant 2, mRNA Homo sapiens uncharaterized LOC112694756 (LOC112694756), transcript variant 1. mRNA ✔Homo sapiens aldolase, fructose-bisphosphate A (ALDOA), transcript variant 4 mRNA Homo sapiens aldolase, fructose-bisphosphate A (ALDOA), transcript variant 3. mRNA 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 Download GenBank 100 ✓
Descriptions Graphic Summary Alignments Sequences producing significant alignments ✔select all 100 sequences selected Description hypothetical protein AB205_0025660 [Lithobates catesbeianus] ✔ALDOA isoform 20 [Pongo abelii] ✔ALDOA isoform 18 [Pongo abelii] aldolase A [Ovis aries] ✔aldolase, fructose-bisphosphate A [Homo sapiens] ✔aldolase, fructose-bisphosphate A [Homo sapiens] aldolase [Elachura formosa] ALDOA ALDOA ✔ALDOA isoform 15 [Pongo abelii] isoform 19 [Pongo abelii] aldolase aldolase, C [Hypophthalmichthys molitrix] fructose-bisphosphate A [Homo sapiens] aldolase, fructose-bisphosphate A [Homo sapiens] ALDOA protein [Homo sapiens] ✔fructose-bisphosphate aldolase A-like [Vulpes lagopus] fructose-bisphosphate aldolase A [Cebus imitator] > > > > > > > > > > > > > > > Taxonomy Download Select columns Show 100 ✓ GenPept Graphics Scientific Name Max Total Query E Score Score Cover value Per Acc. Ident Len Accession ▼ ♥ w V 4 5e-18 100.00% 146 PIO11897.1 7e-18 100.00% 140 PNJ33385.1 Lithobates catesbeianus Pongo abelii Pongo abelii Ovis aries Homo sapiens Homo sapiens Elachura formosa 81.6 81.6 97% 80.9 80.9 97% 81.3 81.3 97% 80.9 80.9 97% 80.5 80.5 97% 81.3 81.3 97% 80.5 80.5 97% Pongo abelii 82.0 82.0 97% Pongo abelii 80.5 80.5 97% Hypophthalmichthys m... 81.3 81.3 97% Homo sapiens 80.5 80.5 97% Homo sapiens 81.6 81.6 97% Homo sapiens 82.4 82.4 97% Vulpes lagopus 82.4 82.4 97% Cebus imitator 80.9 80.9 97% 8e-18 100.00% 162 PNJ33383.1 8e-18 100.00% 139 AAF60276.1 9e-18 100.00% 140 KA12578073.1 9e-18 100.00% 162 KA12578071.1 1e-17 100.00% 147 NXD32569.1 1e-17 100.00% 204 PNJ33381.1 1e-17 100.00% 155 PNJ33384.1 1e-17 100.00% 189 ADF97603.1 1e-17 100.00% 155 KA12578072.1 1e-17 100.00% 204 KA12578074.1 2e-17 100.00% 260 AAH00367.2 3e-17 100.00% 269 XP 041618395.1 3e-17 100.00% 193 XP 037591036.1