You run a DEAE-cellulose column to purify anions from a biological sample. Which approach, from the options listed below

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answerhappygod
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You run a DEAE-cellulose column to purify anions from a biological sample. Which approach, from the options listed below

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You run a DEAE-cellulose column to purify anions from a
biological sample. Which approach, from the options listed below,
would you take to elute the bound fraction?
Apply a buffer of decreased anionic strength to the column.
Apply a buffer of increased anionic strength to the column.
Apply the equilibration buffer to the column.
Apply a buffer of increased pH to the column.
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