-Preparation of 100 mL of 0.050 M phosphate buffer pH 6.5 from 0.50 M stock solution pH 6.5 -Preparation of 100 mL of 0.

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Preparation Of 100 Ml Of 0 050 M Phosphate Buffer Ph 6 5 From 0 50 M Stock Solution Ph 6 5 Preparation Of 100 Ml Of 0 1 (79.27 KiB) Viewed 57 times

-Preparation of 100 mL of 0.050 M phosphate buffer pH 6.5 from 0.50 M stock solution pH 6.5 -Preparation of 100 mL of 0.0050 M phosphate buffer pH 6.5 from 0.50 M stock solution pH 6.5 -Preparation of 100 mL of 0.050 M phosphate buffer pH 6.5 from the given solids -Preparation of 100 mL of 0.0050 M phosphate buffer pH 6.5 from the given solids

Table 7b-2. pH change. P COM Parameters Distilled water Samples 0.050 M phosphate buffer 6.65 pH before addition of NaOH pH after addition of NaOH pH before addition of HCI pH after addition of HCI 6.71 9.48 6.54 4.02 0.0050 M phosphate buffer 6.48 6.57 6.35 6.21 6.71 6.30 6.25 Table 7b-3. Buffer capacity. Parameters Volume of phosphate buffer, mL Initial pH Final pH Final buret reading (Vt), mL Initial buret reading (Vi), mL Samples 0.050 M phosphate buffer 0.0050 M phosphate buffer 35.0 35.0 6.31 6.52 7.31 7.52 42.10 42.80 35.00 42.10 Preparation of Buffers 1. To prepare the phosphate buffers solutions from a stock solution, use Eqn 7b-7. CiV1 = C2V2 (Eqn 7b-7) 2. To prepare the phosphate buffers from solid NaH2PO4-2H20 and solid Na2HPO4-2H20, use Eqn 7b-8, Eqn 7b-9, and Eqn 7b-10. pH = pka + log [A] [HA] (Eqn 7b-8) no. of moles of component mole fraction of component = total no. of moles (Eqn 7b-9) mass of component = (Mbuffer)(Vbuffer)(MMcomponent)(mole fraction component) (Eqn 7b-10)