Question 14: Consider the information in Figure 14. Which one of the following factors does not influence the mass of an

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Question 14 Consider The Information In Figure 14 Which One Of The Following Factors Does Not Influence The Mass Of An 1 (30.97 KiB) Viewed 12 times

Question 14 Consider The Information In Figure 14 Which One Of The Following Factors Does Not Influence The Mass Of An 2 (124.52 KiB) Viewed 12 times

Question 14: Consider the information in Figure 14. Which one of the following factors does not influence the mass of analyte that can be extracted into the SPME coating? O The temperature at which the extraction is done. The distribution of the analyte between the absorptive coating and the sample. O The volume of the absorptive coating. The concentration of the analyte in the sample. O The mass of analyte depends on all the above-mentioned factors.
Figure 14 The following method was used to determine the levels of nicotine (1) in the urine of smokers. A 1.00 cm' sample of urine was placed in a 12 cm³ vial containing 0.7 g Na,CO, powder. After 5.00 ug of the internal standard 5-aminoquinoline (2) was injected, the vial was capped with a rubber septum. The vial was heated to 80°C for 20 minutes before an SPME needle was passed through the septumn. The fibre was left in the headspace for 5 minutes. Then volatile substances were desorbed from the fibre at 250°C for 10 minutes in the GC injection port. During this time the column was held at 60°C while the analyte and internal standard were trapped at the beginning of the column. The column temperature was then raised to 260°C at 25°C-min'. The eluate was monitored by El-MS with SIM at "/, 84 for nicotine and at "/, 144 for the internal standard. All values are averages of duplicate measurements. NH₂ Calibration y = 0.0064x + 0.0222 R' = 0.9956 A (nicotine) A (5-aminoquinoline) 50 100 150 250 Concentration (ug.L') 1.6 14 1.2 1 0.8 0.6 0.4 0.2 D 0 200