2. PCR Products Design a primer that will successfully allow DNA polymerase to work on the top DNA template strand (the
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2. PCR Products Design a primer that will successfully allow DNA polymerase to work on the top DNA template strand (the
2. PCR Products Design a primer that will successfully allow DNA polymerase to work on the top DNA template strand (the dashed lines in the template sequence represent a long sequence of unspecified bases in the target gene)? DNA Template 5'ATTCGG-...GTCCAG3 3'TAAGCC-..._CAGGTC5' 3. What would happen to the DNA sequence if the PCR mixture contained ddNTPs and not dNTPs? Hint: recall the structural differences between ddNTPs and dNTPs and the way that DNA polymerase works.
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