You Have Just Sequenced A Short Segment Of Dna You Want To Determine If This Dna Sequence Could Encode A Protein 5 Tt 1 (122.4 KiB) Viewed 11 times
You have just sequenced a short segment of DNA. You want to determine if this DNA sequence could encode a protein. 5' TTAATGCGCTActatagctacAACTCATCCACT 3' TEMPLATE Comp: 3(AATTACGCGATgatatcgatg TTGAGTAGGTGA 5' CODING 1. Fill in the complimentary strand (Comp:) label 5' and 3' ends 2. Find the longest open reading frame (ORF) and circle the start and stop codons 3. Label boxes on right side, which strand will be the template and which will be non- template (coding) when the longest ORF is transcribed. 4. Transcribe this ORF into a mature mRNA, 5' to 3. mRNA: 5 AUG AGU UUA GCG CAU UAA 3' 5. Translate this mRNA into amino acids (AA), amino (N) to carboxyl (C) termini. AA: N- MET SER LEU ALA HIS -C
You have just sequenced a short segment of DNA. You want to determine if this DNA sequence Start codon- ATG could encode a protein. Stop Codon- TAA, TAG,TGA STOP 3 START 5’ TTAATGCGCTActatagctacảACTCATCCACT 3' TEMPLATE 3' AATTACGCGATgatatcgatg TTGAGTAGGTGA 3' 1 STOP Comp: CODING 4 START 1. Fill in the complimentary strand (Comp:) label 5' and 3' ends 2. Find the longest open reading frame (ORF) and circle the start and stop codons 3. Label in the boxes on right side, which strand will be the template and which will be non- template (coding) when the longest ORF is transcribed.
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