A group of curious students designed a chimeric protein, which contains an ER signal sequence at its N-terminal region,

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A group of curious students designed a chimeric protein,
which contains an ER signal sequence at its N-terminal region, a
nuclear localization signal in the middle and a GFP (green
fluorescent protein) at its C-terminal end. They generated an
expression plasmid and then transfected the plasmid into cultured
cells. Using a fluorescence microscope, where do you think this
GFP-fusion protein is likely to be found at the normal
condition?
in the cytoplasm
in the ER
in the nucleus
in the ER and in the nucleus
The translocation which activates the Abl kinase has
been succesfully targeted by
using a specific small molecule inhibitor called
Gleevec.
blocking G-protein coupled receptors with small molecule
inhibitors.
using the tyrosine kinase inhibitor
Trastuzamab.
Using CRISPR-Cas9 to target the Bcr-Abl
gene.
You analyzed a protein sequence and found there is a
transmembrane alpha helix and a nuclear localization signal. Where
do you expect the later step of synthesis of this protein
(translation) to occur? and where do you expect to find this
protein inside cells?
Cytosol, the ER
Cytosol, the nucleus
Rough ER, the ER
Rough ER, the nucleus
Please answer all questions no need for explanation
ASAP.