- My Unknown Name Litmus Milk Gelatin Indole Methyl Red Voges Prosk Lysine Decarbox Ornithine Decarbox Citrate Nitrate P 1 (384.34 KiB) Viewed 13 times
AND 134. Please Use the possible bacteria unknown sheets.
My unknown # Name Litmus Milk Gelatin Indole Methyl red Voges Prosk Lysine Decarbox Ornithine Decarbox Citrate Nitrate PPA Urea 11 * Starch KIA H2S Glucose Lactose Mannitol Sucrose Rafinose Bile Esculin Comments + + + AA- + Rod Rod a? a V --? + + NC +? + IB + +? + + ܟ ܟ + IN a + +? + -? + + V + + 16 f 1 + +w + - - + + + + +? +- ? V - ? - - -? Rod >0.9 Rod >0.9 Rod <0.9 Rod Rod Cocci Rod Rod Cocci KA- ??- ??- AA- AA- ??- AA- AA- +V +,G -? +, G +, G +,G + + a + + + - + + + + + Uzol + a,B,X + V- - + - Ac-R AcG-R +1+1+1+1+ IG + + + + - +? -+? +, G +, G +,G V V green sheen EMB f + + +- + + + RCGS AACR + ay + Cocci + Y a А Yellow clny, opt. @ 30°C Use this chart with caution. Aeromonas hydrophila Alcaligenes faecalis Bacillus cereus Bacillus megaterium Bacillus subtilis Citrobacter freundii Enterobacter aerogenes Enterococcus faecalis Escherichia coli Klebsiella pneumoniae Lactococcus lactis Micrococcus luteus Moraxella catarrhalis Mycobacterium phlei Mycobacterium smegmatis Neisseria sicca Proteus mirabilis Proteus vulgaris Pseudomonas aeruginosa Serratia marcescens Shigella flexneri Sporosarcina ureae Staphylococcus aureus Staphylococcus epidermidis Staphylococcus saprophyticus Streptococcus agalactiae Streptococcus pyogenes Cocci Rod +WIN + +? + + Growth at 52°C No Growth at 52°C + +? + +WIN - IN? а - - Y B - G f + - + -+ + + ??- + ??- + +? + KA+ +,G KA+ +,G +? KNC+G KA- +,G +w? KA-? + G + + + + + - Rod Cocci Rod Rod Rod Rod Rod Cocci Cocci + K K G a + --+ + + + - - - + + + + f + IV + + +-+ + + + G +? -+ + optimal growth @ 42°C red colonies @ RT sucrose (-) durham yellow, tube prpl optimal @ 30°C Yellow colonies + N α +? - - + + B +? + Ac - +-? +-+ +- ??- ??- + If - ? + A + +-?-+-+ - - 1 + + Cocci Cocci + IN + + +? Y? B + + Cocci Cocci + IB afn = aerobic, facultatively anaerobic or anaerobic ??- A = acid N= hou. K = alkaline W = weak V = variable ? = I am unsure of results ......... " + - or - + = First result is expected result with lab strain
Student name: 4th and 5th Unk Dichotomous Key Page 1 Aeromonas hydrophila Alcaligenes faecalis Bacillus cereus Bacillus megaterium Bacillus subtilis Citrobacter freundii Enterobacter aerogenes Enterococcus faecalis Escherichia coli Klebsiella pneumoniae Lactococcus lactis Micrococcus luteus Moraxella catarrhalis Mycobacterium phlei Mycobacterium smegmatis Neisseria sicca Proteus mirabilis Proteus vulgaris Pseudomonas aeruginosa Serratia marcescens Shigella flexneri Sporosarcina ureae Staphylococcus aureus Staphylococcus epidermidis Staphylococcus saprophyticus Streptococcus agalactiae Streptococcus pyogenes (+) Cell morphology: Bacillus (-) Go to Page 5 Go to Page 2
Student name: 4th and 5th Unk Dichotomous Key Page 2 (+) Starch Hydrolysis (-) (+) Lysine Decarboxylase (-) (+) Endospore Stain (-) Go Page 4 Go to Page 3 Go to Page 3 Go to Page 4
Student name: 4th and 5th Unk Dichotomous Key Page 3 (+) Nitrate Test (-) (+) ZN Acid Fast Stain (-) (+) Raffinose PR Broth (-) (+) Growth at 52 deg C (-)
Student name: 4th and 5th Unk Dichotomous Key Page 4 (+) Urea Hydrolysis (-) (+) Voges - Proskauer Test (-) (+) Indole (- Lactose (+) PR broth (-) (+) Methyl Red (+) Citrate Test (-) (+) Urease (-) (+) Nitrate (-)
Student name: 4th and 5th Unk Dichotomous Key Page 5 (+) Catalase Test (-) (+) Glucose PR Broth (-) (+) Starch Hydrolysis (-) Go to Page 6 (+) Capsule Stain (-) (+) Bile Esculin (-) Go to Page 6
Student name: 4th-nd 5th Unk Dichotomous Key o Title] Page 6 (+) Mannitol PR Broth (-) (+) Gram Stain (-) Urea (+) Hydrolysis (-) (+) Nitrate Test (-) (+) Nitrate Test (-)
Unknown 4 (A) Your Unknown number: 104 Rod Growth B f + +-+ . + + Cell Shape: Approximate Cell Width (um) Gram Growth on MacConkey Agar: Hemolysis a, f, n Motility SIM Spores Acid fast Capsule Catalase Oxidase Litmus Milk Gelatin Indole Methyl red Voges Prosk Citrate Lysine Decarbox Ornithine Decarbox Nitrate PPA Starch Urea KIA H2S Glucose Lactose Mannitol Sucrose Raffinose Bile Esculin + +/- -/+ + + + KA+ +,G
Unknown 5 (B) Your Unknown number: 134 Rod + No growth a f + + +? Cell Shape: Approximate Cell Width (um) Gram Growth on MacConkey Agar: Hemolysis a, f, n Motility SIM Spores Acid fast Capsule Catalase Oxidase Litmus Milk Gelatin Indole Methyl red Voges Prosk Citrate Lysine Decarbox Ornithine Decarbox Nitrate +W + -? + PPA + ??- + Starch Urea KIA H2S Glucose Lactose Mannitol Sucrose Raffinose Bile Esculin Variable +