Blast Search Below are two protein sequences in FASTA format. Perform a BLAST search against the SWISS-PROT database. NO

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Blast Search Below Are Two Protein Sequences In Fasta Format Perform A Blast Search Against The Swiss Prot Database No 1 (95.16 KiB) Viewed 56 times

Blast Search Below are two protein sequences in FASTA format. Perform a BLAST search against the SWISS-PROT database. NOTE: Make sure to select the correct database (swissprot) and alignment method (blastp) in the drop-down menus! Q1: Which functions would you assign to these two proteins based on your BLAST results? Q2: Try using different substitution matrices when performing the BLAST searches. How does this affect the expectation scores? (For instance, note the E-values for the database hit "ADH3_ECOLI" using BLOSUM45, BLOSUM62, and BLOSUM80 and compare). >SOME_PROTEIN STAGKVIKCKAAVLWEVKKPFSIEDVEVAPPKAYEVRIKMVAVGICRTDDHVVSGNLVTP LPVILGHEAAGIVESVGEGVTTVKPGDKVIPLFTPQCGKCRVCKNPESNYCLKNDLGNPR GTLQDGTRRETCRGKPIHHFLGTSTESQYTVVDENAVAKIDAASPLEKVCLIGCGFSTGY GSAVNVAKVTPGSTCAVFGLGGVGLSAVMGCKAAGAARIIAVDINKDKFAKAKELGATEC INPQDYKKPIQEVLKEMTDGGVDFSFEVIGRLDTMMASLLCCHEACGTSVIVGVPPASQN LSINPMLLLTGRTWKGAVYGGFKSKEGIPKLVADFMAKKFSLDALITHVLPFEKINEGFD LLHSGKSIRTVLTF >LAST_ECOLI MRITIILVAPARAENIGAAARAMKTMGFSDLRIVDSQAHLEPATRWVAHGSGDIIDNIKV FPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKSSWMSHAALVFGREDSGLT NEELALADVL TGVPMVADYPSLNLGQAVMVYCYQLATLIQQPAKSDATADQHQLQALRER AMTLLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRLLHDIEKNITK