Mo 3 Mutations Rll 1 And Rll 2 Are Used To Infect E Coli K At High Mo I And The Progeny Phage Collected The Progeny P 1 (555.61 KiB) Viewed 19 times
Mo 3 Mutations Rll 1 And Rll 2 Are Used To Infect E Coli K At High Mo I And The Progeny Phage Collected The Progeny P 2 (344.64 KiB) Viewed 19 times
mo 3. Mutations rll-1 and rll-2 are used to infect E. coli K at high mo.i and the progeny phage collected. The progeny phage are then used to reinfect cells of E. coli K but at low m.o.i.. The lysate is diluted and plated on both E. coli B and E. coli K. Do you expect more plaques on strain B, on strain K, an equal amount on both or is it impossible to tell? (select one) E. coli B. E. coli K Equal on both Can't tell 4. E. coli strain B is doubly infected with two rll mutants of phage T4.0.1 ml of a 10^8 dilution of the progeny is plated on E. coli B and 0.1 ml of a 1045 dilution of the progeny is plated on E. coli K. 37 plaques appeared on strain B, 9 on strain K. Calculate the recombination frequency between these two mutations.
Testing Your Knowledge of Benzer's Experiments 1. Mutations rll-1 and rll-2 are known to fall into the rllA and rllB cistrons (genes) respectively. Mutations rll-1 and rll-2 are used to infect E. coli B at high m.o.i and the progeny phage collected. The progeny phage are used to infect cells of E. coli Kat high m.o.i. and the resulting phage are collected. These progeny are diluted and plated on both E. coli B and E. coli K. Do you expect more plaques on strain B, on strain K, an equal amount on both or is it impossible to tell? (select one) E. coli В. E. coli K Equal on both Can't tell 2. Cells of E. coli B are infected simultaneously with phage strains rll-1 and rll-2 at a high moi. The progeny phage are collected and used to infect cells of E. coli K at low m.o.i.. The resulting phage are diluted and plated on both E. coli B and E. coli K. Do you expect more plaques on strain B, on Strain K, an equal amount on both or is it impossible to tell? (select one) E. coli B. E. coli K Equal on both Can't tell
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