- 4 A B A Geneticist Want To Isolate The Gene From A Tick That Encodes The Anti Coagulant Protein Found In Its Saliva W 1 (112.73 KiB) Viewed 14 times
4. a) b) A geneticist want to isolate the gene from a tick that encodes the anti-coagulant protein found in its saliva which prevents human blood clotting, with the purpose of transferring this gene to bacteria and producing the anti-coagulant for medicinal purposes. Should the geneticist construct a tick genomic or cDNA library? Motivate fully. Which tick tissue will be most suitable as source of the nucleic acids used for library construction? Explain. A partial amino acid sequence from the tick anti-coagulant protein is: ..... Tyr-Met-Ser-Arg-Phe-Val-Tyr-Lys-His-Cys-Met-Leu-Ile-Arg-Thr-Pro ..... You wish to make a set of DNA probes to screen your tick library for the clones containing the sequence that encodes this protein. Your probes should be 15 nucleotides in length. c) 2 Which amino acids in the protein should be used to construct the probes so that the least degeneracy results (consult the genetic code)? How many different probes must be synthesized to be certain that you will find the correct sequence that specifies the protein? Give the nucleotide sequence of any one of these probes.