Q1: Give one lysis buffer commonly used for western blotting experiments and include its components. As soon as lysis oc

[answerhappygod]

Q1: Give one lysis buffer commonly used for western
blotting experiments and include its components.
As soon as lysis occurs, proteolysis, dephosphorylation
and denaturation begin. These events can be slowed down
considerably if samples are kept on ice or at 4°C at all times and
appropriate inhibitors (protease and phosphatase) are added fresh
to the lysis buffer.
Q2. Why are inhibitors necessary for sample
preparation?
Q3: To make sure that you used a similar amount of
samples, what important step should be done before proceeding the
electrophoresis stage?
Q4. Why is it necessary to store the prepared lysates at
a low temperature?
Q5. Give one condition for the denaturation step
employed in blotting
Q6. Give the main two compounds responsible for the
formation of the gels