a) Discuss the statement of "SFC is better than HPLC" (note: only discuss on SFC). (4 marks) b) Supercritical fluids hav

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A Discuss The Statement Of Sfc Is Better Than Hplc Note Only Discuss On Sfc 4 Marks B Supercritical Fluids Hav 1 (60.99 KiB) Viewed 5 times

a) Discuss the statement of "SFC is better than HPLC" (note: only discuss on SFC). (4 marks) b) Supercritical fluids have a few special properties in chromatographic separations. List THREE properties. (3 marks) ) c) A mixture of four compounds as shown in Figure 1 was analyzed using supercritical fluid chromatography (SFC) with carbon dioxide as the supercritical fluid mobile phase. 0 H.C 'N CH, N H.C CH N N 'N HN NH ತ್ವ : 2 : 0 N N N N 0 N N H CH CH CH Caffeine Theophylline Theobromine Uracil Figure 1 Compounds that present in the sample mixture The compounds were separated using column 4.6 mm x 150 mm, packed with 3.5 pm RX-SIL (silica column). A temperature of 50 °C and a pressure of 150 bar was used. The flow was set at 4 mL/min.