CHC BIOL 130 Lab 0.1- DNA Forensic Analysis revised Fall 2021 Name Sec Yi Worksheet I: PAPER MURDER RFLP DNA & RESTRICTI

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: Chc Biol 130 Lab 0 1 Dna Forensic Analysis Revised Fall 2021 Name Sec Yi Worksheet I Paper Murder Rflp Dna Restricti 1 (52.97 KiB) Viewed 53 times

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CHC BIOL 130 Lab 0.1- DNA Forensic Analysis revised Fall 2021 Name Sec Yi Worksheet I: PAPER MURDER RFLP DNA & RESTRICTION ENZYMES Suspect #1 DNA, cut with BamHI, summary of numerical data: CGGATCCGAATTCTAAGAATTCTGGATCCGAATTCGGGATCCAGGATCCTGGAATTCG GCCTAGGCTTAAGATTCTTAAGACCTAGGOTTAAGCCCTAGGTCCTAGGACCTTAAGC Suspect #2 DNA, cut with BamHI, summary of numerical data: GGATCCGGAATTCTAAGAATTCGGATCCGAATTCGGATCCCTGAATTCACGGATCCTT CCTAGGCCTTAAGATTCTTAAGCCTAGGCTTAAGCCTAGGGACTTAAGTGCCTAGGAA Suspect #3 DNA, cut with BamHi, summary of numerical data: AGGAATTCGAGAATTCGGATCCTTGGATCCGAATTCTACGGATCCGAATTCGGATCCC TCCTTAAGCTCTTAAGCCTAGGAACCTAGGCTTAAGATGCCTAGGOTTAAGCCTAGGG Suspect #1 DNA, cut with EcoRI, summary of numerical data: CGGATCCGAATTCTAAGAATTCTGGATCCGAATTCGGGATCCAGGATCCTGGAATTCG GCCTAGGCTTAAGATTCTTAAGACCTAGGCTTAAGCCCTAGGTCCTAGGACCTTAACC Suspect #2 DNA, cut with EcoRI, summary of numerical data: GGATCCGGAATTOTAAGAATTCGGATCCGAATTCGGATCCCTGAATTCACGGATCCTT CCTAGGCCTTAAGATTCTTAAGCCTAGGCTTAAGCCTAGGGACTTAAGTGCCTAGGAA Suspect #3 DNA, cut with EcoRI, summary of numerical data: AGGAATTCGAGAATTCGGATCCTTGGATCCGAATTCTACGGATCCGAATTCGGATCCC TCCTTAAGCTCTTAAGCCTAGGAACCTAGGCTTAAGATGCCTAGGCTTAAGCCTAGGG Page 3 of 7
Name Sec Worksheet II: PAPER MURDER RFLP ANALYSIS 5 4 4 4 4 4 3 3 3 3 3 2 2 2 2 2 1 1 1 1 1 0 0 0 0 0 8 6 4 2 0 8 6 4 2 0 8 6 4 2 0 8 6 4 2 0 8 6 4 2 DNA nucleotide count BamHI DNA from the blood splash Suspect #1's DNA Suspect #2's DNA Suspect #3's DNA o 5 0 4141414 6 4 2 O 8 6 3 3 2 2 2 0 8 6 4 1 1 1 1 0 0 0 0 2 DNA nucleotide count EcoRI DNA from the blood splash Suspect #1's DNA Suspect #2's DNA Suspect #3's DNA Questions:
D Question 5 1 pts Enter the fragment lengths for Suspect #2 DNA cut with EcoRI. Enter the data in sequence from left to right, separated by commas and a space: #,#,#,#,# Question 6 1 pts Enter the fragment lengths for Suspect #3 DNA cut with EcoRI. Enter the data in sequence from left to right, separated by commas and a space: #, #, #, #, #
Question 16 5 pts In a murder case, a crime lab identified one suspect as a match for both the BamHI and the EcoRI analysis. Would it be helpful to do further DNA testing with a third restriction a enzyme? Why or why not? ( This is a hypothetical question, using a scenario that may or may not match your worksheet results.)
D Question 17 5 pts In a murder case, a crime lab's run of three suspects found that none matched the crime scene DNA in the BamHI and the EcoRI analysis. Would it be helpful to do further DNA testing with a third restriction enzyme? Why or why not? ( This is a hypothetical question, using a scenario that may or may not match your worksheet results.)