The next step is to place the fragments onto a gel and then apply a current. The fragments will move through the gel, bu

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The Next Step Is To Place The Fragments Onto A Gel And Then Apply A Current The Fragments Will Move Through The Gel Bu 1 (497.22 KiB) Viewed 55 times

The next step is to place the fragments onto a gel and then apply a current. The fragments will move through the gel, but smaller fragments will move farther in the gel. DNA has a negative charge, so it will move toward the positive end of the fragment when electricity is applied. Once the fragments have been separated, the gel is stained so that the DNA bands will be visible under a UV light. The bands will look like a barcode. A marker can also be used to indicate how long each fragment is. Marker Sample A Sample B Sample C The image shows a marker with fragments of known lengths and three samples. Length of DNA fragment (base pairs) 1,000 bp 1) How many fragments were in sample A? 900 bp 2) Which sample had the shortest fragment ? 800 bp 700 bp 3) Carefully examine sample C and determine the length of each fragment (bp). 600 bp 500 bp 400 bp 4) Discuss WHY samples would have fragments of different lengths. 300 bp 200 bp 100 bp 5) Would you expect different samples that came from the same person, such as skin cells and blood, to have the same number and length of fragments? Why or why not? 6) Would samples that were digested with EcoRI have a different pattern than the same sample digested with Smal? Explain your answer.