K2 Streaking For Isolation To Make A Pure Culture Post Lab Report What You Will Submit In One Document Uploaded To Can 1 (53.21 KiB) Viewed 44 times
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K2 (Streaking for Isolation to Make a Pure Culture) Post-Lab Report What you will submit in ONE document uploaded to Canvas: Results and observations, including Drawings of both of your plates in color Written lab notes (1.e. colony counts, number of different colonies on each plate) For each unique type of colony, describe the 6 colony morphology characteristics for the different isolated colonies on your plates (refer to Kleyn Fig 5.2) Required photos of plates (Period 2 and 3) • Laboratory Report (below) - not the report in the Kleynlab manual! o o 0 1. Answer the questions that follow (Note that this lab exercise and the Kleyn lab manual can help you answer some of these questions): How many different types of yeast colonies did you isolate on the yeast/PDA plate? 2. How many different types of bacterial colonies did you isolate from the yogurt/MRS plate? 3. Why is it necessary to use a new sterile loop for each region on an isolation streak plate? 4. Provide two reasons why plates should be inverted during incubation 1) 2) 5. Define "subculture". Why is it necessary to subculture the colony to be sure it is a pure culture? Describe at least two different ways you could end up with a contaminant if you do not properly use aseptic technique when inoculating/streaking a plate. 7. Discussion on a separate piece of paper): Consulting the results in your lab notebook from this exercise. address the following topics: a. Did one sample (yeast or yogurt) have more types of organisms? Explain how you can tell. Why you think one product has more species in it than the other. b. Did you find isolated colonies? If not, why not? How could you improve your technique to get isolated colonies on future streak plates? c. Did you find contamination anywhere? If so, in your discussion/conclusion describe what you think caused the contamination (when did the contamination happen during your procedure). d. Explain the importance of aseptic technique and why/how you will use aseptic technique to culture organisms in this class, and why it's important for handling patient samples.
MB 2) 4729/22POA Yeast I PS
MRS W12 Yogurt мн va CEC
MR 58/CE POA yeast any
MU MRS spetza Yogurt I
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