I Need Help With The Following Questions 1 (115.88 KiB) Viewed 24 times
I Need Help With The Following Questions 2 (101.03 KiB) Viewed 24 times
In the two scatterplots (two-dimensional dot plots) below, the first image (left) shows the forward versus side scatter of white blood cells from human blood. You can see that a gate has been placed around the lymphocytes (shows cells in red). The second plot (right) is looking at the expression of two cell surface markers, on the gated cells only (lymphocytes only, not on monocytes or neutrophils) - this is an example of sequential gating (something that you will do as you analyse your data in the Week 11 prac). Looking at the plot on the right, what percentage of lymphocytes are cytotoxic T cells? You do not need to include the % symbol in your answer. Human Blood Leukocytes Scatter-Gated Human Lymphocytes 은 1023 OL 102 44% Neutro 1% -phils g CD4 Side Scatter 31% Monocytes 101 Sdatter Gate Lymphocytes 23% 0 1023 10 10 10 10 104 Forward Scatter CD8 Answer:
In Section C, you are using B220 and CD3 as markers of B and T cells. When you analyse your flow cytometry data (such as in the figure below), the cells in which quadrant would be your B cells? Hint: this is not a trick question! 10 1 4 10" B220 10- 02 3 0102 10 10" 10 CD3 Select one: O a. 2 Ob. 2 and 4 O c. 1 and 2 O d. 3 O e. 1 and 3 Of. 1 O g. 3 and 4 Oh. 1 and 4 OI. 4
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