You Wish To Clone A Fragment Of Dna Into A Plasmid Vector The Plasmid Has A Multiple Cloning Site With The Following Re 1 (232.56 KiB) Viewed 47 times
You Wish To Clone A Fragment Of Dna Into A Plasmid Vector The Plasmid Has A Multiple Cloning Site With The Following Re 2 (232.56 KiB) Viewed 47 times
You wish to clone a fragment of DNA into a plasmid vector. The plasmid has a multiple cloning site with the following restriction enzyme sites: 5 EcoRI / Hindill / Clal / Bglii / Kpnl 3' The DNA fragment has BamHl sites at both the 5' and 3' ends. What enzyme would you cut the plasmid with? Explain your answer using figure 1 below. EcoRI 5 GAATTC3 3' CTTAAG 5 Hindill S'ALGCTT3' 3' TTCGAA5 Kpnl 5'GGTACC 3 3' CATGG 5 Bglli 5' AGATCT 3 з' TCTAQAS BamHI 5 CGATCC 3' з' CCTAdG 5' Clal 5' ATCGAT3 3' TAGTA5 Figure 1. Restriction enzyme recognition sites and positions where the DNA is cut. A human variable number of tandem repeats (VNTR) locus contains a tandem repeat (TTA), where n may be between 5 and 15. How many alleles of this locus are possible in the human population?
You wish to clone a fragment of DNA into a plasmid vector. The plasmid has a multiple cloning site with the following restriction enzyme sites: 5 EcoRI / Hindill / Clal / Bglii / Kpnl 3' The DNA fragment has BamHl sites at both the 5' and 3' ends. What enzyme would you cut the plasmid with? Explain your answer using figure 1 below. EcoRI 5 GAATTC3 3' CTTAAG 5 Hindill S'ALGCTT3' 3' TTCGAA5 Kpnl 5'GGTACC 3 3' CATGG 5 Bglli 5' AGATCT 3 з' TCTAQAS BamHI 5 CGATCC 3' з' CCTAdG 5' Clal 5' ATCGAT3 3' TAGTA5 Figure 1. Restriction enzyme recognition sites and positions where the DNA is cut. A human variable number of tandem repeats (VNTR) locus contains a tandem repeat (TTA), where n may be between 5 and 15. How many alleles of this locus are possible in the human population?
Join a community of subject matter experts. Register for FREE to view solutions, replies, and use search function. Request answer by replying!