CATCH UP TEST 3 [30] QUESTION 1 a) The separation of components in a mixture is done using different chromatographic tec

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Catch Up Test 3 30 Question 1 A The Separation Of Components In A Mixture Is Done Using Different Chromatographic Tec 1 (98.61 KiB) Viewed 53 times

CATCH UP TEST 3 [30] QUESTION 1 a) The separation of components in a mixture is done using different chromatographic techniques. Discuss the major differences between thin layer and partition chromatography (3) b) The van Deemter equation relates the resolving power (HETP, height equivalent to a theoretical plate) of a chromatographic column to the various flow and kinetic parameters which cause peak broadening. With the aid of a fully labeled diagram, show all the terms involved in the van Deemte plot well as the combined effect. (3) c) In a chromatographic separation, compound M eluted from 10 cm column at 5.7 minutes. The peak width at the base of the peakwas 0.5 minute. i) Calculate he plate number (2) ii) Calculate the plate height in mm (2) iii) Is it a bigger or smaller H that is recommended, give a reason for your answer. (2) d) Explain how will you determine or confirm whether the two peaks are fully resolved or not and how can you manipulate it. (2) (14) Page 2 of 6 2/6