ASK CongSha a Suedes imported from Youtube Maps New P Question Completion Status QUESTION 3 10 points An unknown protein

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: Ask Congsha A Suedes Imported From Youtube Maps New P Question Completion Status Question 3 10 Points An Unknown Protein 1 (30.67 KiB) Viewed 77 times

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ASK CongSha a Suedes imported from Youtube Maps New P Question Completion Status QUESTION 3 10 points An unknown protein was determined using the following method Bradford method Reagents Stock Bradford reagent: Dissolve 100 mg Coomassie Brilliant Blue 250 in a mixture consisting of 100m of 85% phosphoric acid, 50ml of 5% ethanol and Som 1M NaOH. Store at 4" un precipitation occurs, at which point it is discarded Working Bradford reagent. Prepare fresh by duting tomt of stock Bradford reagent to 250ml with distled water Stock bovine serum wbumin (BSA) solution (16mm Dissolve 0.29 BSA in 20ml dissiled water Working BoA concentration range: 0,5 - 125 mm Tube 4 Tube 5 Unknown 0. O. Oom Method 1. Prepare the following test tubes Table 1 Preparation of test tubes for the Bradford method Blank Tube 1 Tube 2 Tube 3 Distilled H20 0. 0.mi O. 0.25 mg im BSA 0.1 mi 0.5 mgimi BSA 0.1 0.75 mg BSA 0.1 1.0 mgimi BSA 1.25 mgimi OSA Unknown protein Working 4.Om 4 40 4. 0.1 mi Omni 02 4. Omi 4 Om 4. Om Citi
Apos SK Conding She a suggested Imper M News Question Completion Status Stock bovine serum albumin (BSA) solution (10mg/ml Dissolve 0.29 BSA in 20mcistilled water Working BSA concentration range: 0,5 - 1,25 mg/ml 0 Method 1. Prepare the following test tubes Table 1: Preparation of test tubes for the Bradford method Blank Tube 1 Tube 2 Tube 3 Tube 4 Tube 5 Unknown Distilled H20 1.0 mi Omni 0.mi 09mi 0.9m 0.9mi 0.8m 0.25 mgimi BSA 0.1 mi 0.5 mg/ml BSA 0.1 ml 0.75 mg/ml BSA 0.1 mi 1.0 mgimi BSA 0.1 ml 1.25 mgimi BSA 0.1ml Unknown 02ml protein Working 4.Om 4.Omi 4.Om 4.0ml 4 Omi 4.Omi 4.Omi Bradford 2. Aner each addition of the working Bradford reagent, thoroughly mix the resultant solution a incubate the solution at room temperature for 5 min. Read the absorbance at 105nm of the blank and test solutions Calculate the protein concentration (in mg/ml) of an unknown sample with an absorbancy value of 0.123 using the standard curve below.
> SK Coulting Sha.. Suggested Sites Imported from YouTube Maps New Translate Question Completion Status: Cite the protein concertation in mg mayor an unknown Sample with an orancy Value O.TZS using the sandale curve now 15 14 13 13 1 10 DR Absorbancy 595m 07 03 05 04 03 N OD 01 02 03 44 12 13 1 15 16 06 07 08 09 10 BSA concentration /