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QUESTION 1 Both Biuret and Bradford methods was used to determine unknown concentrations of both BSA and Blg. The methods followed is described in detail in the Background section The following results was obtained: Table 1: Absorbance at 595nm obtained for different concentrations of protein standards using the Bradford method [Protein] 0.00 0.125 0.25 0.50 0.75 1.00 (mg/ml) 1.50 2.00 Unknown Abs, BSA 0 0.130 0.350 0.620 0.870 1.120 1.400 1.60 1.050 Abs. Big 0 0.080 0.130 0.360 0.520 0.650 0.850 1.080
18 1.7 1.6 1.5 Abs. BSA Abs. Big 1.4 13 1.2 1.1 10 Absorbancy (595nm) 0.9 0.8 0.7 0.6 0.5 0.4 0.3 0.2 0.1 0.0 0.00 0.25 0.50 1.50 1.75 2.00 0.75 1.00 1.25 Protein concentration (mg/ml)
Table 2: Absorbance at 540nm obtained for different concentrations of protein standards using the Biuret method [Protein] 0.00 0.125 0.25 0.50 0.75 1.00 (mg/ml) 1.50 2.00 Unknown Abs. BSA 0 0.026 0.045 0.075 0.111 0.128 | 0.172 0.218 0.26 Abs. Blg 0 0.024 0.052 0.092 0.136 0.162 0.223 0.264
0.30 0.28 0.26 0.24 -Abs. BSA -Abs, Big 0.22 0.20 0.18 0.16 bsorbancy (540nm) 0.14 0.12 0.10 0.08 0.06 0.04 0.02 0.00 0.00 0.50 1.50 0.25 1.75 2.00 0.75 1.00 1.25 Protein concentration (mg/ml)
Which standard curve would you use to determine the concentration of the Unknown protein sample? O Blg standard curve using the Biuret method Big standard curve using the Bradford method BSA standard curve using the Biuret method BSA standard curve using the Bradford method