- Question 1 Both Biuret And Bradford Methods Was Used To Determine Unknown Concentrations Of Both Bsa And Blg The Method 1 (29.53 KiB) Viewed 103 times
- Question 1 Both Biuret And Bradford Methods Was Used To Determine Unknown Concentrations Of Both Bsa And Blg The Method 2 (55.62 KiB) Viewed 103 times
- Question 1 Both Biuret And Bradford Methods Was Used To Determine Unknown Concentrations Of Both Bsa And Blg The Method 3 (17.79 KiB) Viewed 103 times
- Question 1 Both Biuret And Bradford Methods Was Used To Determine Unknown Concentrations Of Both Bsa And Blg The Method 4 (49.87 KiB) Viewed 103 times
- Question 1 Both Biuret And Bradford Methods Was Used To Determine Unknown Concentrations Of Both Bsa And Blg The Method 5 (17.35 KiB) Viewed 103 times
18 1.7 1.6 1.5 Abs. BSA Abs. Big 1.4 13 1.2 1.1 10 Absorbancy (595nm) 0.9 0.8 0.7 0.6 0.5 0.4 0.3 0.2 0.1 0.0 0.00 0.25 0.50 1.50 1.75 2.00 0.75 1.00 1.25 Protein concentration (mg/ml)
Table 2: Absorbance at 540nm obtained for different concentrations of protein standards using the Biuret method [Protein] 0.00 0.125 0.25 0.50 0.75 1.00 (mg/ml) 1.50 2.00 Unknown Abs. BSA 0 0.026 0.045 0.075 0.111 0.128 | 0.172 0.218 0.26 Abs. Blg 0 0.024 0.052 0.092 0.136 0.162 0.223 0.264
0.30 0.28 0.26 0.24 -Abs. BSA -Abs, Big 0.22 0.20 0.18 0.16 bsorbancy (540nm) 0.14 0.12 0.10 0.08 0.06 0.04 0.02 0.00 0.00 0.50 1.50 0.25 1.75 2.00 0.75 1.00 1.25 Protein concentration (mg/ml)
Which standard curve would you use to determine the concentration of the Unknown protein sample? O Blg standard curve using the Biuret method Big standard curve using the Bradford method BSA standard curve using the Biuret method BSA standard curve using the Bradford method