You have an idea for a vaccine to prevent Group A Strep (GAS) infections. You know that Streptococcus bacteria are fasti

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You have an idea for a vaccine to prevent Group A Strep (GAS)
infections. You know that Streptococcus bacteria
are fastidious organisms, and you don't expect to be able to
produce your protein of interest in large quantities in S.
pyogenes. Based on your knowledge of GAS, design a recombinant
vaccine candidate to protect against GAS infection
using E. coli as your recombinant protein
production organism. Your choice of GAS antigen should be a
bacterial protein that is not expressed by humans. Your GAS
protein(s) of choice will be expressed in E.
coli and then purified for use in vaccine
production. To express your protein in E.
coli, you need to clone the gene(s) of interest into a
plasmid under the control of the
Plac promoter. Create
and upload a diagram that shows each step of your design strategy
and cloning process. Start with getting your gene of interest out
of S. pyogenes and end with your selection and
screening process in E. coli. Be sure to include all
of the following:
- What GAS protein(s) will be expressed by your
recombinant E. coli?
- Show all components that need to be present on your plasmid
for replication, selection, screening, and for regulation by the
Plac promoter.
- How do all of the parts come together?
- A selection mechanism to ensure that only
recombinant E. coli expressing your plasmid can
grow. Any selection mechanism is ok. Indicate what media needs to
be used and what you expect to see.
- A screening mechanism to show that your gene(s) of interest is
being expressed. Indicate what media needs to be used and what you
expect to see.