- 1 (209.1 KiB) Viewed 175 times
foci formation in response to genotoxic agents inducing
interstrand DNA crosslinks. (A,C) U2OS and HeLa cells were
transfected with siControl, siPML, or siATR and treated with
1 µM mitomycin C (MMC) for 24 h. FANCD2 protein expression was
determined though Western blotting of cell lysates
from U2OS and HeLa. Long form (L) of FANCD2 represents
mono-ubiquitination and short form (S) is non-ubiquitinated
FANCD2. (B,D) FANCD2 foci formation in U2OS and HeLa cells treated
with siControl or siPML under 1 µM MMC exposure
for one day. Representative immunofluorescence images and their
quantification. Over 100 nuclei were counted for statistics.
Data represent the mean ± SD from three independent experiments
(*** p < 0.001). (E,F) Prolif
A.explain the image above (only A and C)?
b. how does Depletion of PML Causes Impairment of Damage-Induced
FANCD2 Mono-Ubiquitination?
A с HeLa U2OS siPML siContol SIATR siContol SiPML SiATR + MMC MMC L S FANCD2 FANCD2 0 c-Tubulin a-Tubulin B D U2OS DAPI FANCD2 Hela DAPI FANCD2 Merge PML Merge PML MMC - siControl MMC siControl MMC+ MMC + MMC- MMC- SiPML SiPML MMC + MMC + U2OS Hela *** *** *** 100 *** 100 *** 80 FANCD2 OPML 80 FANCD2 OPML 60 60 Cells with >5 foci (%) 40 40 20 20 MMC- MMC + siControl MMC- MMC + SIPML MMC- MMC+ siControl MMC- MMC + siPML Figure 1. Cont.