- Question 22 As Part Of The Positional Cloning Of A Human Disease Gene Finding That The Dna Sequence Of A Candidate Gene 1 (111.44 KiB) Viewed 262 times
QUESTION 22 As part of the positional cloning of a human disease gene, finding that the DNA sequence of a candidate gene is consistently altered in affected individuals and not in unaffected ones is good evidence that your candidate gene is indeed the one responsible for the disease phenotype. As part of your research, you examine the DNA sequence from an exon of one of your favorite candidate genes in several breast cancer patients and several unaffected members of the same families. The sequences shown are coding strand sequences with reading frame indicated by spacing. Sequence differences from the consensus human genome are indicated in bold. The codon table is provided below. normal GAT CAG CCT GAC CTT GAA GCC normal GAT CAA CCT GAC CTT GAG GCC normal GAT CAA CCA GAC CTT GAA GCC affected: GAT CAG CCT GAC CTT GAA GCC affected: GAT CAA CCT GAC CTT GAG GCC Based on the sequence region shown, is this a good candidate gene for hereditary breast cancer? وأعف | Second U A ulucu w UGU LUC UCC UNC Ocom / UUA JUCA SA SUGAS LUG Low UCG LAG SUGG CUU COU CAU Cou Cuc COC CAC ) CGC CUA CCA PICA с CGA GUG coa CAG COG AUU) ACU MAU AGU AUC ACC AG ARC AGC AUA The ACA AAA AUG MNG AGANG AGG GUU GOU QUCsc. GOC GAC) GOC GUA local GAA GOA GUS GOO GAG GO AG yes no cannot determine from the data QUESTION 23 You have identified a Drosophila gene that is expressed exclusively in the odd-numbered "stripes in the cellular blastoderm. Assuming that this gene is not redundant, what would be the most likely phenotype cause by a loss-of- function mutation in this gene? O an embryo missing odd numbered segments O an embryo missing larval segments 3-10 O an adult fly with a second pair of wings instead of halteres O an embryo with two anterior ends O an embryo missing even-numbered segments