4. You decide to characterize protein A encoded by Gene A in muscle cells by making cDNA using the mRNA derived from the

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4 You Decide To Characterize Protein A Encoded By Gene A In Muscle Cells By Making Cdna Using The Mrna Derived From The 1 (55.18 KiB) Viewed 219 times

4. You decide to characterize protein A encoded by Gene A in muscle cells by making cDNA using the mRNA derived from the wild-type allele of Gene A and by adding Oligo-dT primers. The Gene A cDNA has the recognition sites for restriction enzymes X and R as shown below. 5) 311 DNA GORA بي إلا Direction of transcription a. From the choices below, choose the sequences that are a part of the Gene A but are NOT contained in the corresponding cDNA. (3 marks) Promoter Exons Introns Enhancers 3'UTR b. You want to clone the cDNA for Gene A into the plasmid below that has recognition sites for restriction enzymes Y, R, X and A as shown in the figure. Note: A vertical line (1) represents the cutting site for each restriction enzyme. Which enzyme(s) would you use to cut the plasmid that would allow directional cloning and expression of Gene A from the bacterial promoter and why? (6 marks) Bacterial promoter R Size: 3Kb Kn Bacterial Ori Amp