d. Another group is competing with you. Although they have the bacterial clone that contains the recombinant plasmid wit

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D Another Group Is Competing With You Although They Have The Bacterial Clone That Contains The Recombinant Plasmid Wit 1 (66.39 KiB) Viewed 266 times

d. Another group is competing with you. Although they have the bacterial clone that contains the recombinant plasmid with Gene A in correct orientation they cannot express Gene A in bacteria. To understand what the issue is they decide to PCR amplify and sequence Gene A isolated from their bacterial clone (mutant) and compare it with the sequence of Gene A that you published (wild-type). Shown below is the sequence flanking the mutant allele of Gene A. 5'GAAATS Gene A CGACTT3' Top strand 3' CTTTAC CCTGAA5. Bottom strand Choose from the list below the primer pair which will be best for amplifying Gene A by PCR reaction? (3 marks) 1: 5'GAAATC3' 2: 5'TTCAGG3 3:5'CTTTAG3' 4:5’AAGTCC3' e. Sequencing results show that the mutant version has one point mutation compared to the wild-type version of Gene A. Shown below is a portion of the fluorescence dideoxy- sequencing gel that gives the sequence of the mRNA like strand/ non- template strand of the DNA. Write the sequence of the mRNA that corresponds to the mutant allele of Gene A. (5 marks) Fluorescent dideoxy- CROTERAPIE 3 Fluorescent dideoxy-G Fluorescent dideox-I Fluorescent dideoxy-A 5 47