1. (a) Describe the preparation of 250 ml of a 200 mM sodium phosphate buffer, pH 7.5, containing 600 mM NaCl and 0.02%
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1. (a) Describe the preparation of 250 ml of a 200 mM sodium phosphate buffer, pH 7.5, containing 600 mM NaCl and 0.02%
1. (a) Describe the preparation of 250 ml of a 200 mM sodium phosphate buffer, pH 7.5, containing 600 mM NaCl and 0.02% (w/v) NaN3. Show all calculations. [M-values (g/mol): See Table 2; NaN3 = 65.1; NaCl = 58.44). [5] (b) Explain how you would prepare 500 ml of a 150 mM HCl solution. The following information is given on the bottle of HCl: M, = 36.46; 37% (v/v); density = 1.16 g/ml. [4]
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