- Question Completion Status 2 7 9 10 Take Readings And Record Each Of The Control Curve Concentrations For The Colorea M 1 (62.01 KiB) Viewed 55 times
Question Completion Statuss 8 10 7 6 5 3 4 next repeat the data collection process for the experimental solutions (called the "unknowns") use the CONTROL CURVE data (or least-square linear regression equation of the CONTROL CURVE) to find the real concentrations of the colored molecule being measured in the experiment after the first reading be sure to clean and dry the cuvette, and then again after each successive reading, so as not to dilute the sample solutions with the previous sample solution construct the control curve for the colored molecule and check to see that the graph is linear
Remains Question Completion Status: 1 2 3 4 8 9 10 (5 points) When performing an experiment that generates a colored product, we often use a sp ctrophotometer to measure the intensity of color absorption as the marker for the reaction under study. However in setting up the experimental conditions, we need to be careful with the intensity of the color when it is read/recorded in the spectrophotometer. The reason is that the absorption curve is linear only when the absorption reading is between 0.2 and 0.8 on the deflection scale. Above that range the curve saturates off and the reading is no longer reliable, and below that range linearity/sensitivity is unreliable. ================== The reaction in this experiment relies upon accurately measuring the concentration of the colored compound by establishing a control curve before testing the unknowns. In the BlackBoard video associated with this lab the lab tech went through all of the steps needed to perform this lab successfully.