2. Plot rate (umol/min) versus E in milligrams. The changes in absorbance per minute must be converted to micromoles, us
Posted: Tue Nov 16, 2021 6:30 am
- 2 Plot Rate Umol Min Versus E In Milligrams The Changes In Absorbance Per Minute Must Be Converted To Micromoles Us 1 (109.51 KiB) Viewed 54 times
Enzyme Volume (mL)
Start
15”
30”
45”
60”
75”
90”
105
0.067
0.03
0.10
0.15
0.21
0.27
0.32
0.38
0.44
0.133
0.07
0.21
0.33
0.45
0.57
0.68
0.79
0.89
0.200
0.11
0.33
0.52
0.69
0.86
0.99
1.04
1.04
0.267
0.14
0.44
0.69
0.90
1.03
1.03
1.03
103
0.333
0.19
0.52
0.82
103
1.03
1.03
1.03
1.03
Substrate Volume (mL)
Start
15”
30”
45”
60”
75”
90”
105
33
0.0
0.03
0.06
0.10
0.13
0.16
0.19
0.22
67
0.0
0.03
0.06
0.11
0.16
0.20
0.25
0.30
133
0.02
0.06
0.12
0.17
0.23
0.28
0.33
0.38
267
0.01
.07
0.13
0.19
0.25
0.31
0.37
0.43
533
0.0
0.05
0.12
0.18
0.25
0.31
0.37
0.43
667
0.01
0.09
0.16
0.23
0.30
0.37
0.43
0.48
2. Plot rate (umol/min) versus E in milligrams. The changes in absorbance per minute must be converted to micromoles, using Beer's law and the extinction coefficient for dopachrome. This is similar to the calculations we did earlier for LDH: (A A/A min) umol product/min = X (106 uM/M) X 0.003 L 3600 M-1