2. Plot rate (umol/min) versus E in milligrams. The changes in absorbance per minute must be converted to micromoles, us

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2 Plot Rate Umol Min Versus E In Milligrams The Changes In Absorbance Per Minute Must Be Converted To Micromoles Us 1 (109.51 KiB) Viewed 53 times

Do not need to plot, just need values to generate a plot
Enzyme Volume (mL)
Start
15”
30”
45”
60”
75”
90”
105
0.067
0.03
0.10
0.15
0.21
0.27
0.32
0.38
0.44
0.133
0.07
0.21
0.33
0.45
0.57
0.68
0.79
0.89
0.200
0.11
0.33
0.52
0.69
0.86
0.99
1.04
1.04
0.267
0.14
0.44
0.69
0.90
1.03
1.03
1.03
103
0.333
0.19
0.52
0.82
103
1.03
1.03
1.03
1.03
Substrate Volume (mL)
Start
15”
30”
45”
60”
75”
90”
105
33
0.0
0.03
0.06
0.10
0.13
0.16
0.19
0.22
67
0.0
0.03
0.06
0.11
0.16
0.20
0.25
0.30
133
0.02
0.06
0.12
0.17
0.23
0.28
0.33
0.38
267
0.01
.07
0.13
0.19
0.25
0.31
0.37
0.43
533
0.0
0.05
0.12
0.18
0.25
0.31
0.37
0.43
667
0.01
0.09
0.16
0.23
0.30
0.37
0.43
0.48
2. Plot rate (umol/min) versus E in milligrams. The changes in absorbance per minute must be converted to micromoles, using Beer's law and the extinction coefficient for dopachrome. This is similar to the calculations we did earlier for LDH: (A A/A min) umol product/min = X (106 uM/M) X 0.003 L 3600 M-1