Restriction Enzymes Number of Base Pairs (BP) PUC19 cut with Avall (A) 250 BP - 2,500 BP PUC19 cut with Pvull (P) 300 BP - 2,300 BP PUC19 cut with Avall and Pvull (AP) (A800 BP - 1,200 BP) (P250 BP - 300 BP) uncut pUC19 (UP) 2,700 BP DNA ladder (L) 250 - 3000BP
Restriction Enzymes Distance Traveled (mm) PUC19 cut with Avall (A) 4.7 mm 4 mm PUC19 cut with Pvull (P) PUC19 cut with Avall and Pvull (AP) uncut pUC19 (UP) 4.7 mm 1 mm DNA ladder (L) 4.7 mm
Answers to boldface questions. Graph of log(fragment size) vs. distance traveled, including a linear regression line and it equation. A map of PUC 19 showing the Avall and Pvull restriction sites and the fragment sizes.
Restriction Enzymes Number of Base Pairs (BP) PUC19 cut with Avall (A) 250 BP - 2,500 BP PUC19 cut with Pvull (P) 300 BP
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Restriction Enzymes Number of Base Pairs (BP) PUC19 cut with Avall (A) 250 BP - 2,500 BP PUC19 cut with Pvull (P) 300 BP
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