Cellular Respiration Can Be Studied In Isolated Mitochondria By Measuring Oxygen Consumption Under Different Conditions 1 (76.35 KiB) Viewed 27 times
Cellular respiration can be studied in isolated mitochondria by measuring oxygen consumption under different conditions. If an investigator adds 0.01 M sodium malonate to actively respiring mitochondria that are using pyruvate as fuel source, respiration soon stops and a metabolic intermediate accumulates. Draw the structure of this metabolic intermediate. Please draw the intermediate in its ionized form. Select Erase Draw Rings More / |||||| H Cl N S
Why does this metabolic intermediate accumulate? Oxidative decarboxylation of malonate forms the metabolic intermediate. Malonate blocks substrate-level phosphorylation, leading to an accumulation of the substrate. Malonate activates the enzyme that forms the intermediate, leading to its accumulation. Competitive inhibition of an enzyme by malonate causes the intermediate to accumulate. Why does adding malonate to isolated mitochondria stop oxygen consumption? A block in the citric acid cycle stops formation, which stops oxygen consumption. Aside from removal of the malonate, what can overcome this inhibition of respiration? the regeneration of NAD+ the addition of excess citrate synthase
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