HCHÖNHCH- & NIG 1. BrCN 2. H₂O NGHENIGH ON HNCC R CH₂ R' R CH₂ R' CH₂-S-CH₂ CH₂-OH Proteins can be cleaved specifically

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Hchonhch Nig 1 Brcn 2 H O Nghenigh On Hncc R Ch R R Ch R Ch S Ch Ch Oh Proteins Can Be Cleaved Specifically 1 (34.43 KiB) Viewed 35 times

HCHÖNHCH- & NIG 1. BrCN 2. H₂O NGHENIGH ON HNCC R CH₂ R' R CH₂ R' CH₂-S-CH₂ CH₂-OH Proteins can be cleaved specifically at the amide bond on the carboxyl side of methionine residues by reaction with cyanogen bromide (above). C by a four-stage mechanism. The third stage is an hydrolysis reaction that cleaves the peptide chain. At the end of this stage, the carts former methionine becomes part of a lactone (cyclic ester) ring. Draw curved arrows for the step that cleaves the peptide chain. Arrow-pushing Instructions X :0: :OH 0: Ở NHCHC NHCH CHÍNH, CHO 0-H - R H₂C LO: R' H -NHCHC -