14-44. This problem requires Beer's law from Chapter 18. The oxidized form (Ox) of a flavoprotein that functions as a on

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14 44 This Problem Requires Beer S Law From Chapter 18 The Oxidized Form Ox Of A Flavoprotein That Functions As A On 1 (130.55 KiB) Viewed 43 times

14-44. This problem requires Beer's law from Chapter 18. The oxidized form (Ox) of a flavoprotein that functions as a one-electron reducing agent has a molar absorptivity (ε) of 1.12 × 10¹ M¯¹ cm¯¹ at 457 nm at pH 7.00. For the reduced form (Red), ε = - 3.82 × 10³ M-¹ cm at 457 nm at pH 7.00. -1 Ox+e Red E° =-0.128 V The substrate (S) is the molecule reduced by the protein. Red + S Ox+S¯ Both S and S are colorless. A solution at pH 7.00 was prepared by mixing enough protein plus substrate (Red + S) to produce initial concentrations [Red] = [S] = 5.70 × 10-5 M. The absorbance at 457 nm was 0.500 in a 1.00-cm cell. a. Calculate the concentrations of Ox and Red from the absorbance data. b. Calculate the concentrations of S and S¯. c. Calculate the value of E°/ for the reaction S + e-S¯.