- Assignment2 A Series Of Standard Calibration Solutions Were Prepared In 100 Ml Volumetric Flask By Reacting Standard Sol 1 (51.98 KiB) Viewed 40 times
- Assignment2 A Series Of Standard Calibration Solutions Were Prepared In 100 Ml Volumetric Flask By Reacting Standard Sol 2 (32.95 KiB) Viewed 40 times
ASSIGNMENT2 A series of standard calibration solutions were prepared in 100 mL volumetric flask by reacting standard solutions of phosphate with molybdenum(VI) and reducing the phosphomolybdic acid complex to produce the characteristic blue color. The concentration of stock solution was 100 mg/L. The absorbance, A were measured using colorimetric spectrometer as shown in the below table. A calibration curve was plotted against the concentration of phosphorous. Volume of Phosphorous Concentration of Absorbance from stock solution (mL) phosphorus (mg/L) 1 0.001 5 0.086 10 0.173 25 0.403 50 0.794 Unknown sample 0.201 le *
ASSIGNMENT2 g L 1. Calculate the concentration of phosphorus prepared in the series of calibration curve. 2. Assuming a linear relationship between the variables, determine the linear least- squares equation and correlation coefficient. 3. Determine the standard error of the intercept. 4. Find the standard deviation of the intercept. 5. Determine the limit of detection and limit of quantification. 6. Calculate the phosphorous concentration in the urine sample.