- Bovine Insulin 1 Treatment With Fdnb 2 Acid Hydrolysis 3 Oxidation Of Cysteines 4 Paper Electrophoresis Results Sa 1 (87.35 KiB) Viewed 39 times
- Bovine Insulin 1 Treatment With Fdnb 2 Acid Hydrolysis 3 Oxidation Of Cysteines 4 Paper Electrophoresis Results Sa 2 (23.48 KiB) Viewed 39 times
Bovine Insulin 1. Treatment with FDNB 2. Acid hydrolysis 3. Oxidation of cysteines 4. Paper Electrophoresis: Results: Sanger found three DNP-amino acids: DNP - glycine, DNP-phenylalanine, and E-DNP lysine. He concluded that bovine insulin has two polypeptide chains, one with Gly at its aminoterminus and one with Phe at its amino terminus. One of the chains contains the ε-DNP lysine, not at the amino - terminus. a) Explain how Sanger's result support his conclusions. b) Are the results consistent with the known structure of insulin ?
A chain B chain NH, Gly NH Phe Val lle Val Aen Glu Gin 6 His 5 Gin -Cys Leu Cys S S Cys S Ala Gly S Ser Ser 10 Val 30 His Cys Leu Ser Val Leu Ghu Тут Ala 13 Gin 13 Leu Leu Tyr Leu Glu Aan Val Tyr Cys 20 Cys 30 Gly Asn Glu Сою Arg Gly Phe 4 Phe Тут Thr Pro Lys Ala doo