B And Pet 32cc 32an Shown Wit Ans Pet 32b Is Ract 1 Bp From Cach 8 Pet 32c Is A Lbp To Each Site E 1 (57.54 KiB) Viewed 43 times
B And Pet 32cc 32an Shown Wit Ans Pet 32b Is Ract 1 Bp From Cach 8 Pet 32c Is A Lbp To Each Site E 2 (13.86 KiB) Viewed 43 times
b(+) and pET⋅32cc+ 32an+)( shown) wit! ans- pET-32b(+) is : ract 1 bp from cach 8. pET−32c(+) is a lbp to each site \& except for EcoR V
4.1. How many fragments will form if pET32a(+) is digested simultaneously with Sacl and BcA. [1] 4.2. Write down the base pairs that result at the 5′ and 3′ ends of the shortest fragment [3] 4.3. Restriction enzymes often recognize palindromic sequences. What is a palindromic sequence? [2] 4.4. Give three restriction enzymes found on the pET32(a+)multiple cloning site. [3]
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