Assays used to assess lymphocyte function generally attempt to answer one of the following questions: Do the B or T cell

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Assays used to assess lymphocyte function generally attempt to answer one of the following questions: Do the B or T cell

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Assays Used To Assess Lymphocyte Function Generally Attempt To Answer One Of The Following Questions Do The B Or T Cell 1
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Assays used to assess lymphocyte function generally attempt to answer one of the following questions: Do the B or T cells respond normally to mitogenic stimuli that activate cells to undergo a proliferative response? Does mitogenic or antigen-driven stimulation result in antibody production for T cells? Does mitogenic or antigen-driven stimulation result in cytokine production for T cells? Does mitogenic or antigen-driven stimulation result in antibody production for B cells?
The following is true of mitogens mitogens Mitogens that selectively activate B cells will cause polyclonal stimulation of B cells. Non-specific stimuli, do not act through the antigen-specific receptor Lectins, including concanavalin A (Con A) and phytohemagglutinin (PHA), are very effective T-cell mitogens. Pokeweed mitogen (PWM) is a lectin with potent mitogenic properties PWM stimulates polyclonal activation of both B and T cells. Concanavalin A and phytohemagglutinin, are very effective B-cell mitogens
T-cell assays are Cytotoxicity assays measures the activity of CD4 +T cells against radiolabeled cells Assays that measure T helper cell function focus on helper activity for B cells can be used to measure the helper properties of CD4+T cells Cytotoxicity assays measures the activity of CD8+ cytotoxic T cells against radiolabeled cells Antibody-dependent cell-mediated cytotoxicity is studied by culturing radiolabeled cells with NK cells and cell-specific antibodies
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