A Restriction Enzyme Ecorl Cuts A Plasmid At The Three Sites Shown Above Distances Between The Sites Are Indicated A 1 (252.69 KiB) Viewed 51 times
A Restriction Enzyme Ecorl Cuts A Plasmid At The Three Sites Shown Above Distances Between The Sites Are Indicated A 2 (479.77 KiB) Viewed 51 times
A Restriction Enzyme Ecorl Cuts A Plasmid At The Three Sites Shown Above Distances Between The Sites Are Indicated A 3 (311.63 KiB) Viewed 51 times
A restriction enzyme, EcoRL, cuts a plasmid at the three sites shown above. Distances between the sites are indicated. a. What is the total size of the plasmid (in kb)? By adding up the distances between a and b,b and c, as well as c and a, the total distance is 10 kb. b. If the plasmid is completely digested with EcoRL, three fragments will be generated, and these will migrate as shown on the gel below:
b. If the plasmid is completely digested with EcoRL, three fragments will be generated, and these will migrate as shown on the gel below:
If the plasmid is INCOMPLETELY digested, and cuts only at sites a and b, draw how the resulting fragments will migrate in the gel shown below (put them in the correct position, but also indicate their sizes to the right of the bands):
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