What is the problem associated with the use of linkers for putting sticky ends to a blunt ended molecule?

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answerhappygod
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What is the problem associated with the use of linkers for putting sticky ends to a blunt ended molecule?

Post by answerhappygod »

a) High ambient temperature requirements
b) Possible cleavage of the DNA molecule itself
c) High cost of synthetic linkers
d) Low compatibility of linkers
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